Embryology research services with modern methods such as IVF

Embryology services provided by Histogenotech

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کشت جنینی
Embryology and the science of reproduction is one of the most prolific and rapid growing medical sciences in the last three decades. Gamete preparation, culture, successful embryo formation in the laboratory, long-term storage or proper transfer to the uterus at the appropriate time are examples of the most sensitive and essential activities in the field of infertility treatment.
Thus, the Histogenotech laboratory is ready to provide the following services to researchers.
و محیط کشت اسپرم

Embryology study services

Freezing or embryo cryopreservation to keep the fetus in liquid nitrogen for a long time at different developmental stages from 2 nuclei to blastocysts is common in assisted reproduction laboratories. Egg freezing requires nutrient culture medium with osmolality and suitable pH of fetal growth to maintain fetal quality. In addition, in order to achieve quality embryos, the use of frozen eggs and sperm in the laboratory can be effective in coordinating ovarian cycles and the readiness of the uterus for embryo transfer. Relying on the knowledge of experienced specialists in the Andarology and Embryology Laboratory of Histogenotech with ART laboratory equipment and specialized culture medium for freezing and thawing embryos, eggs and sperm, high survival results have been observed during the defrost process. It has had significant effects on successful in vitro fertilization in mice.
Sometimes sperm cannot penetrate the outer layer of the egg for various reasons. The outer layer of the egg may be thick or the surface receptors may be damaged or the sperm may not be able to swim. In these cases, a procedure called intracytoplasmic sperm injection (ICSI) is performed in conjunction with in vitro fertilization (IVF) to help fertilize the egg. During ICSI, a sperm is injected directly into the cytoplasm of the egg. There are two ways to fertilize an egg in the laboratory. Conventional method and ICSI. In traditional IVF, 50,000 or more floating sperm are placed next to the egg in a laboratory container. Fertilization occurs when one of the sperm enters the cytoplasm of an egg. In the ICSI process, a small needle called a micropipette is used to inject a sperm into the center of the egg. With traditional IVF, or ICSI, when fertilization occurs, the fertilized egg (now called the embryo) grows in the laboratory for 1 to 5 days before being transferred to the uterus.
In order to achieve quality embryos, sperm and eggs with normal morphology, uniform cytoplasm and healthy DNA are needed. Various environmental factors including environmental stresses in assisted reproductive laboratories, in vitro cell culture and storage processes, freezing and thawing, and in vivo factors including genetic disorders in both males and females can lead to the development of defective embryos or gametes with damaged DNA. There are a variety of laboratory tests in Histogenotech to evaluate apoptosis in germ cells or fertilized embryos that can be effective in diagnosing this problem or designing research studies. These tests include the tunnel test, the annexin test, and the halosperm test.
Antioxidant variables such as catalase (CAT), superoxide dismutase (SOD), GPX, GSH, TAC and oxidative stress variables including ROS and MDA on tissues and germ cells and embryos obtained in various studies have been studied. In fact, the measurement of these oxidative markers and antioxidants and creating research pathways in the study of various additives or herbal and chemical supplements to the culture medium of embryos and germ cells, can be considered in the treatment of ovarian and testicular diseases such as PCO and azoospermia.
Antioxidant variables such as catalase (CAT), superoxide dismutase (SOD), GPX, GSH, TAC and oxidative stress variables including ROS and MDA on tissues and germ cells and embryos obtained in various studies have been studied. In fact, the measurement of these oxidative markers and antioxidants and creating research pathways in the study of various additives or herbal and chemical supplements to the culture medium of embryos and germ cells, can be considered in the treatment of ovarian and testicular diseases such as PCO and azoospermia.
In order to transfer the embryo (embryo transfer) into the uterine tissue in the animal model, it is necessary to create a false pregnancy condition (Pseudo pregnancy). A false pregnancy, clinically called a pseudo pregnancy, assumes that you are expecting a baby when you are not actually carrying a baby. People with miscarriages have pregnancy symptoms but no fetus in the womb. Accordingly, sex hormones are high and the uterine wall is ready to accept the fetus, but there is no fetus. This condition is induced in animals in order to induce pregnancy by using a swab rotation in the vagina of the female animal or placing the female animal next to the male vasectomy animal. By doing this, the IVF embryos are ready to be transferred to the female animal’s body.
Frozen embryos, sperm and eggs
Freezing or embryo cryopreservation to keep the fetus in liquid nitrogen for a long time at different developmental stages from 2 nuclei to blastocysts is common in assisted reproduction laboratories. Egg freezing requires nutrient culture medium with osmolality and suitable pH of fetal growth to maintain fetal quality. In addition, in order to achieve quality embryos, the use of frozen eggs and sperm in the laboratory can be effective in coordinating ovarian cycles and the readiness of the uterus for embryo transfer. Relying on the knowledge of experienced specialists in the Andarology and Embryology Laboratory of Histogenotech with ART laboratory equipment and specialized culture medium for freezing and thawing embryos, eggs and sperm, high survival results have been observed during the defrost process. It has had significant effects on successful in vitro fertilization in mice.
Intracytoplasmic injection (ICSI)
Sometimes sperm cannot penetrate the outer layer of the egg for various reasons. The outer layer of the egg may be thick or the surface receptors may be damaged or the sperm may not be able to swim. In these cases, a procedure called intracytoplasmic sperm injection (ICSI) is performed in conjunction with in vitro fertilization (IVF) to help fertilize the egg. During ICSI, a sperm is injected directly into the cytoplasm of the egg. There are two ways to fertilize an egg in the laboratory. Conventional method and ICSI. In traditional IVF, 50,000 or more floating sperm are placed next to the egg in a laboratory container. Fertilization occurs when one of the sperm enters the cytoplasm of an egg. In the ICSI process, a small needle called a micropipette is used to inject a sperm into the center of the egg. With traditional IVF, or ICSI, when fertilization occurs, the fertilized egg (now called the embryo) grows in the laboratory for 1 to 5 days before being transferred to the uterus.
جنین، اسپرم و تخمک
Frozen embryos,sperm&eggs
ICSI Intracytoplasmic Sperm Injection
ICSI
آپوپتوز در اسپرم2
Evaluation of apoptosis
بررسی استرس اکسیداتیو در تخمدان
Design & check vector construction
Evaluation of apoptosis in sperm, eggs and embryos
In order to achieve quality embryos, sperm and eggs with normal morphology, uniform cytoplasm and healthy DNA are needed. Various environmental factors including environmental stresses in assisted reproductive laboratories, in vitro cell culture and storage processes, freezing and thawing, and in vivo factors including genetic disorders in both males and females can lead to the development of defective embryos or gametes with damaged DNA. There are a variety of laboratory tests in Histogenotech to evaluate apoptosis in germ cells or fertilized embryos that can be effective in diagnosing this problem or designing research studies. These tests include the tunnel test, the annexin test, and the halosperm test.
Evaluation of oxidative stress in ovarian, testicular, sperm, egg and fetal tissues
Measurement of oxidative stress and types of oxidants and oxygen oxidants (ROS) in ovarian and testicular tissues and in male and female germ cells is one of the most basic tests of research studies and knowing the amount of these compounds plays an essential role in infertility disorders. The presence of poor quality embryos in clinical laboratories can be due to the imbalance between antioxidant and oxidant compounds that can be measured by ELISA and flow cytometry in Histogenotech laboratories.
Investigation of the concentration of antioxidants in tissues and cells
Antioxidant variables such as catalase (CAT), superoxide dismutase (SOD), GPX, GSH, TAC and oxidative stress variables including ROS and MDA on tissues and germ cells and embryos obtained in various studies have been studied. In fact, the measurement of these oxidative markers and antioxidants and creating research pathways in the study of various additives or herbal and chemical supplements to the culture medium of embryos and germ cells, can be considered in the treatment of ovarian and testicular diseases such as PCO and azoospermia.
Fetal transfer to the uterus in a false pregnancy model
In order to transfer the embryo (embryo transfer) into the uterine tissue in the animal model, it is necessary to create a false pregnancy condition (Pseudo pregnancy). A false pregnancy, clinically called a pseudo pregnancy, assumes that you are expecting a baby when you are not actually carrying a baby. People with miscarriages have pregnancy symptoms but no fetus in the womb. Accordingly, sex hormones are high and the uterine wall is ready to accept the fetus, but there is no fetus. This condition is induced in animals in order to induce pregnancy by using a swab rotation in the vagina of the female animal or placing the female animal next to the male vasectomy animal. By doing this, the IVF embryos are ready to be transferred to the female animal’s body.
غلظت آنتی اکسیدان
Antioxidants concentration
جنین به رحم موش صحرائی
Embryo transfer
You may find the answer to your question

Frequently Asked Questions by Customers

In order to keep the embryo, egg or sperm for a short time in freezing conditions, the best way is to keep it at -80 ° C, but if you need to keep it longer, using liquid nitrogen at -196 ° C can lead to Preserve the genomic structure and morphology of cells. Thus, according to extensive studies, this maintenance method can be used for this purpose for many years by maintaining standard storage conditions. This long-term maintenance, sometimes up to 10 years, has been able to lead to the birth of healthy fetuses without any defects.

Molecular tests, including real-time PCR, ICC and Western Blot or function of some enzymes (ELISA), can be performed on fresh and frozen sperm, fresh eggs and frozen Fused with at least 1, fresh and frozen embryos with at least 1 for some tests. For this purpose, more specialized commercial kits were needed in small quantities, and in larger numbers, some tests can be studied manually.

Ovum, as a female germ cell, has different stages of nuclear maturation. Which, being enclosed inside a follicle and supported by follicular cells, develop through the stimulation of hormones (FSH, LH, Estrogen, Progesterone) during the sexual cycle. Depending on the size of the follicles and the number of rows of follicular cells around the egg, the follicles are divided into Primordial, Primary, Secondary, Pre-Antral, and Graf. On the other hand, the eggs inside the follicles are divided into GV, GVBD, MI, MII based on their nuclear maturation stage. In response to different phases of the sexual cycle at any time, some of the eggs can be in any of the stages.

Performing antioxidant tests or oxidative stress in eggs and fetuses with sperm and ovarian and testicular tissue are different due to the small number of fetal cells and eggs. To perform glutathione peroxidase (GPX), oxidative stress (ROS), and test for mitochondrial activity with JC dye, it is necessary to quantify the activity of the enzyme or other variables mentioned with fluorescent images with Image J software and by imaging. In the case of sperm, ovaries and testes, these assessments are performed by ELISA or flow cytometry or spectrofluorometric. Other tests such as superoxide dismutase, total antioxidant capacity or TAC in both cell and tissue can be performed by ELISA.

In order to develop transgenic animals, it is necessary to inject the desired gene fragment into the male pronuclear by microinjection (ICSI). Since the GFP reporter gene can be expressed alongside the target gene, it can indicate the success of vector function in the fetus. Thus, after embryonic cell proliferation, the target gene is expressed in all embryonic cells. The resulting embryo continues to grow by transferring the embryo to the fallopian tube or directly into the uterus to form a complete transgenic organism.

Due to the progress in the production of egg and embryo extraction kits, working with single cells can be done easily, so to perform Real time PCR technique and study gene expression can be expressed with a single egg or embryo by Single cell PCR method to evaluate the embryo and the egg. On the other hand, in order to perform ELISA tests and oxidative stress tests, these tests can be performed with at least 5 eggs and embryos.

Because many of the steps are almost the same with sperm, embryos and ova. Therefore, performing all steps such as sperm washing, freezing and thawing of sperm, examination of sperm parameters, intracytoplasmic injection of sperm, separation of motile sperm and good quality from other sperms, is similar to the human case. However, due to the high sensitivity of working with eggs and embryos, after learning all the steps of working with eggs and embryos of animals, it is necessary to do 6 months and one year internships in human laboratory and then work confidently with embryos and human eggs.

In this regard, the culture container used is made of polystyrene and with proper adhesion should be used. Thus, it is necessary to use HEPES culture medium in manual environments to create stable buffering conditions. In addition, in order to maintain the proper temperature, it is necessary to use the warm plate to maintain a temperature of 37°C during operation. Culture medium droplets with dimensions of 5 to 10 microliters, which can be increased up to 100 microliters, are used in a contracted manner with a specific rhythm from the beginning of the egg entering the container to the last drop as marked. Mineral Oil is used on the prepared droplets to prevent contamination when the lid of the culture dish is open under the microscope.

Embryology service images

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