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biotechnology
Components and regions of expression vector
Multiple Cloning Site DNA cloning inside a vector involves the binding of the target DNA to the vector DNA. The…
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biotechnology
Components and regions of expression vector (Selection Marker,Ribosome Binding Site)
Ribosome Binding Site After the transcription process of the genes, the translation must be performed by ribosomes. Therefore, there should…
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biotechnology
Components and regions of expression vector (Promoter Region)
Promoter Region The promoter causes the expression of the protein and includes the promoter (the position of RNA polymerase) and…
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biotechnology
Components and regions of expression vector (Replicon Region)
Components of expression vector Replicon Promoter Ribosome Binding Site Selection Marker Multiple Cloning Site Replicon Region 1. Low Copy Number…
Genetics Content
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Different methods for identifying and interpreting incomplete bands of western blot techniques
Different methods for detecting incomplete bands Colorimetric Detection This method is based…
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Paper interpretation and unusual band in Western Blot
Western band Uneven bands These bands can be created as a result…
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The interpretation of paper and unusual bands in Western Blot
Unusual bands Although Western blotting is a relatively simple technique, there may…
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Types of materials and equipment required in Western blot techniques
Buffer and Western paper The basis of protein separation in western blotting…
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Polyacrylamide gel electrophoresis in western blot technique
Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis is used to isolate proteins in…
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protein expression assay by western blot in tissue or cell
Introduction Expression of cell or tissue protein by western blot method Western…
Cellular Content
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Cellular
Definitions of cell differentiation and aging and cell culture media components
Cell culture terms Dedifferentiation In this process, the specific characteristics of the cell are lost…
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Molecular Content
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Molecular
cDNA synthesis from RNA extraction from tissue and cell
cDNA Synthesis Determining the amount of mRNA is essential for accurate examination of genes expression. The basic PCR mechanism involves DNA replication as a nucleic acid pattern but not RNA. Thus, the first step is…
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