Effective factors of medium on cultured cells
The factors affecting the phenotype of the cells are :
- The substrate that the cells grow on (solid: plastic, semi-solid: collagen and agar, liquid: suspension media)
- The degree of contact with other cells
- Physico-chemical and physiological compounds in the environment
- Gases amount in the environment
- Incubation temperature
Most cells isolated from solid tissues grow as single sticky layers, except transformed or non-bed-dependent cells. After the cells are separated from the tissue, they need to be bonded and expand on the substrate to proliferate. Usually, cells stick to the glass or plastic surface. This adhesion is accomplished by the receptors for attachment to the extracellular matrix, which can be said the distribution and expansion of cells are related to produce extracellular matrix proteins and proteoglycans. The matrix is bonded to a superconducting substrate (glass and plastic), then the cells connect to the matrix through binding to specific receptors.
The three main groups of transfusion proteins are involved in cell-cell and cell-matrix attachment and play a role in transmitting the message, which results in the binding of nearby cells.
- Cell-cell adhesion molecules
- Calcium dependent (Cadherin)
- Calcium independent
Note: The cell-matrix interaction is made using integrin, which is made up of two sub-units of alpha and beta and has a different outer surface that causes diversity among them.