RNase inactivation in tissue and cells during RNA extraction

Inactivation of RNase

It is suggested to shake micro-tube vigorously for 20seconds to better separation of the liquid–organic phase and prevent itscontamination with protein and DNA.

 How does RNase in atissue or cell can be deactivated?

Guanidine isothiocyanate present in various reagents, suchas thrysol, is an effective compound in the inactivation of proteins, whichresults in inactivation of the RNase enzyme. In addition, it plays a role inrRNA isolation and ribosomal proteins.

Increase in RNA concentration Glycogen can increase the RNAconcentration. Glycogen is insoluble in alcohol. Thus it increases the RNAdeposition when adding alcohols.

The Point :

Some tissues have glycogen, which improves the process ofextraction of RNA from that tissue. It should be noted that excessive glycogenincreases the risk of extracted RNA (260/230). The appropriate amount is onemicroliter of glycogen at a concentration of 20mg / ml.

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