RNase inactivation during RNA extraction

It is suggested to shake micro-tube vigorously for 20 seconds to better separation of the liquid–organic phase and prevent its contamination with protein and DNA.

How does RNase in a tissue or cell can be deactivated?

Guanidine isothiocyanate present in various reagents, such as thrysol, is an effective compound in the inactivation of proteins, which results in inactivation of the RNase enzyme. In addition, it plays a role in rRNA isolation and ribosomal proteins.

Increase in RNA concentration

Glycogen can increase the RNA concentration. Glycogen is insoluble in alcohol. Thus it increases the RNA deposition when adding alcohols.

The Point :

Some tissues have glycogen, which improves the process of extraction of RNA from that tissue. It should be noted that excessive glycogen increases the risk of extracted RNA (260/230). The appropriate amount is one microliter of glycogen at a concentration of 20mg / ml.

Related posts: RNA Extraction from cells and tissues – Quantitative analysis of the extracted RNA cell or tissue with spectrophotometer