Scientific texts
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Production of recombinant proteins by yeasts
Production of recombinant proteins by yeasts Eukaryotic Expression System of yeast cells Yeasts are monocellular eukaryotic fungi and are often…
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Different types of expression systems of recombinant proteins
Different types of expression systems of recombinant proteins Proteins, as one of the essential macromolecules in the biological world, play…
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cell differentiation Ageing and culture media
cell differentiation Ageing and culture media Cell culture terms: Dedifferentiation In this process, the specific characteristics of the cell are…
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Cell cycle processes and separation
Cell cycle processes and separation The cell cycle consists of four phases: G1, synthesis, G2, and mitosis. The cell cycle…
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Effective factors of medium on cultured cells
Effective factors of medium on cultured cells The factors affecting the phenotype of the cells are : The substrate that…
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equipment and Different cell culture techniques
equipment and Different cell culture techniques Preliminary preparations and initial equipment for cell culture techniques In this section, separating cells…
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cDNA synthesis from RNA extraction from tissue and cell
cDNA synthesis from RNA extraction from tissue and cell Determining the amount of mRNA is essential for accurate examination of…
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Removal of genomic DNA contamination during total RNA extraction
Removal of genomic DNA contamination during total RNA extraction To reduce phenolic contamination, ethanol 75% wash can be repeated until…
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Quantitative analysis of the extracted RNA with spectrophotometer
Quantitative analysis of the extracted RNA with spectrophotometer The concentration and purity of the extracted RNA can be assessed using…
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RNase inactivation during RNA extraction
RNase inactivation during RNA extraction It is suggested to shake micro-tube vigorously for 20 seconds to better separation of the…
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