Molecular
- Molecular
Polymerase Chain Reaction PCR
Polymerase Chain Reaction PCR is a process which during an iterative temperature program a specific part of the genome or…
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Primer design
Primers are short DNA sequences usually composed of 18 to 24 base pair which act as the starting point during…
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cDNA synthesis from RNA extraction from tissue and cell
cDNA synthesis from RNA extraction from tissue and cell Determining the amount of mRNA is essential for accurate examination of…
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Removal of genomic DNA contamination during total RNA extraction
Removal of genomic DNA contamination during total RNA extraction To reduce phenolic contamination, ethanol 75% wash can be repeated until…
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Quantitative analysis of the extracted RNA with spectrophotometer
Quantitative analysis of the extracted RNA with spectrophotometer The concentration and purity of the extracted RNA can be assessed using…
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RNase inactivation during RNA extraction
RNase inactivation during RNA extraction It is suggested to shake micro-tube vigorously for 20 seconds to better separation of the…
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RNA Extraction from cells and tissues
RNA Extraction from cells and tissues RNA extraction is performed to purify the RNA from cell and tissue samples. Due…
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Different methods of detecting incomplete bands in western blot techniques
Different methods of detecting incomplete bands in western blot techniques Colorimetric Detection This method is based on incubating western blot…
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The interpretation of paper and unusual bands in Western Blot
The interpretation of paper and unusual bands in Western Blot Although Western blotting is a relatively simple technique, there may…
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materials and equipment required in Western blot techniques
materials and equipment required in Western blot techniques Buffer and Western paper The basis of protein separation in western blotting…
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